Joe Russell and Jonathan Jacobs at MRIGlobal have been designing and building modular systems for real-time, point-of-care molecular microbiology. Recently developed molecular analysis platforms offer the promise of bringing comprehensive detection, diagnostics, and biosurveillance of emerging infectious diseases to extremely remote and austere locations, completely detached from a traditional brick and mortar laboratory.
- Robust extraction of RNA from mosquito pools for purposes of arbovirus surveillance using Biomeme’s ultra portable Bulk Nucleic Acid Extraction kit (mean RNA concentration from 25 mosquito pool: 1.02 ng/μl).
- Effective detection of low-titer arbovirus (Everglades Virus) from single 25-mosquito sample pool using Biomeme two3 qPCR thermocycler. Results comparable to current ‘gold standard’ qPCR platforms.
- Nanopore sequencing capable of detecting RNA virus from complex sample matrix after eukaryote rRNA depletion and whole transcriptome amplification reaction.
UPDATE We are excited to announce that this work has been featured in the GenomeWeb article, "MRIGlobal Demonstrates Nanopore Sequencing in the Field in Step Toward 'Lab-in-a-Backpack'" by Monica Heger. GenomeWeb is an independent online news organization the serves the global community of scientists, technology professionals, and executives who use and develop the latest advanced tools in molecular biology research and molecular diagnostics. To read the full article on genomeweb follow this link (Subscription required)