--- title: WCS Validates Canine Distemper Virus qPCR Test on Biomeme Platform description: Scientists validate point-of-care testing for Canine Distemper Virus using Biomeme's portable M1 Sample Prep and handheld two3 thermocycler. image: https://blog.biomeme.com/hubfs/waldemar-brandt-nxR7gvSokH8-unsplash-1.png --- [![logo](https://blog.biomeme.com/hubfs/logo.svg)](https://biomeme.com) - [Applications](https://biomeme.com/applications) - [Host Response Rapidly analyze host immune responses](https://biomeme.com/applications/host-response) - [Our Evidence](https://biomeme.com/applications/host-response/our-evidence) - [Infectious Disease Detect and identify infectious agents quickly](https://biomeme.com/applications/infectious-disease) - [Pandemic Preparedness](https://biomeme.com/applications/infectious-disease/pandemic-preparedness) - [Biodefense Secure and reliable testing for biodefense applications](https://biomeme.com/cbrn-solutions) - [Biowarfare Detection Suite](https://biomeme.com/biowarfare-detection-suite) - [Solutions by Sector](https://biomeme.com/solutions-by-sector) - [Researchers Portable and efficient testing for remote areas](https://biomeme.com/applications/researchers) - [Platforms](https://biomeme.com/platforms) - [Franklin ISP](https://biomeme.com/platforms/franklin-isp) - [Franklin](https://biomeme.com/platforms/franklin) - [Our Vision](https://biomeme.com/our-vision) - [One Health](https://biomeme.com/our-vision/one-health) - [Antimicrobial Resistance](https://biomeme.com/our-vision/antimicrobial-resistance) - [Antibiotic Resistance](https://biomeme.com/our-vision/antimicrobial-resistance/antibiotic-resistance) - [Antimicrobial Stewardship](https://biomeme.com/our-vision/antimicrobial-stewardship) - [Host Response](https://biomeme.com/our-vision/host-response) - [Layman's View](https://biomeme.com/our-vision/host-response/understanding-host-response) - [Support](https://help.biomeme.com/) - [About](https://biomeme.com/about) - [Careers](https://biomeme.com/careers) - [Leadership](https://biomeme.com/about/leadership) - [Scientific Advisory Board](https://biomeme.com/scientific-advisory-board) - [Insights](https://blog.biomeme.com/) - [Contact Us](https://biomeme.com/about/contact) [![logo](https://blog.biomeme.com/hubfs/logo.svg)](https://biomeme.com) - [Applications](https://biomeme.com/applications) - [Host Response](https://biomeme.com/applications/host-response) - [Our Evidence](https://biomeme.com/applications/host-response/our-evidence) - [Infectious Disease](https://biomeme.com/applications/infectious-disease) - [Pandemic Preparedness](https://biomeme.com/applications/infectious-disease/pandemic-preparedness) - [Biodefense](https://biomeme.com/cbrn-solutions) - [Biowarfare Detection Suite](https://biomeme.com/biowarfare-detection-suite) - [Solutions by Sector](https://biomeme.com/solutions-by-sector) - [Researchers](https://biomeme.com/applications/researchers) - [Platforms](https://biomeme.com/platforms) - [Franklin ISP](https://biomeme.com/platforms/franklin-isp) - [Franklin](https://biomeme.com/platforms/franklin) - [Our Vision](https://biomeme.com/our-vision) - [One Health](https://biomeme.com/our-vision/one-health) - [Antimicrobial Resistance](https://biomeme.com/our-vision/antimicrobial-resistance) - [Antibiotic Resistance](https://biomeme.com/our-vision/antimicrobial-resistance/antibiotic-resistance) - [Antimicrobial Stewardship](https://biomeme.com/our-vision/antimicrobial-stewardship) - [Host Response](https://biomeme.com/our-vision/host-response) - [Layman's View](https://biomeme.com/our-vision/host-response/understanding-host-response) - [Support](https://help.biomeme.com/) - [About](https://biomeme.com/about) - [Careers](https://biomeme.com/careers) - [Leadership](https://biomeme.com/about/leadership) - [Scientific Advisory Board](https://biomeme.com/scientific-advisory-board) - [Insights](https://blog.biomeme.com/) - [Contact Us](https://biomeme.com/about/contact) # WCS Validates Canine Distemper Virus qPCR Test on Biomeme Platform ![](https://blog.biomeme.com/hubfs/waldemar-brandt-nxR7gvSokH8-unsplash-1.png) **Written By:** Biomeme Staff Ania Tomaszewicz Brown, Denise McAloose, Paul P. Calle, Angelika Auer, Annika Posautz, Sally Slavinski, Robin Brennan, Chris Walzer, and Tracie A. Seimon developed a quantitative RT-PCR assay for Canine distemper virus (CDV) using our handheld two3™ qPCR thermocycler. CDV is a multi-host pathogen that can cause significant mortality in domestic, wild terrestrial and marine mammals. It is a major conservation threat in some endangered species. Infection can result in severe respiratory disease and fatal encephalitis. Biomeme's extraction methodology, lyophilized reagents, and two3™ thermocycler were compared to standard laboratory-based methods to assess sensitivity, efficiency and overall test performance. Key takeaways include: - Results using a positive control plasmid for CDV showed comparable sensitivity (detection of 50 copies) and PCR efficiency between the two platforms, and CDV detection was similar between platforms when tested using a modified live CDV vaccine. - Significantly higher Ct values (average Ct = 5.1 cycles) were observed using the Biomeme platform on known CDV positive animal samples. - CDV detection using the Biomeme platform was similar in 25 of 26 samples from suspect CDV cases when compared to standard virology laboratory testing. - One false positive was observed that was negative upon retest. - Biomeme’s methodology eliminated the need for local or international sample transport typically seen in laboratory-based diagnostics. - Results of their testing suggested that decreased diagnostic sensitivity (higher Ct values) relative to laboratory-based methods was observed using animal samples, so careful validation and optimization are essential. (Biomeme’s two3™ qPCR thermocycler has since been retired and replaced with our latest [Franklin™ qPCR thermocycler](https://info.biomeme.com/mobile-qpcr-thermocyclers)) [Read the full research article](https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0232044#abstract0) over at [PLOS ONE](https://journals.plos.org/plosone/) to learn how portable qPCR platforms can empower biologists and wildlife health professionals in remote and low-resource settings to greatly improve their understanding of CDV disease ecology and associated conservation threats in wildlife. Biomeme is supporting a number of Wildlife Conservation Society initiatives, including Border Control and eDNA Detection of Endangered Species: [The Future of Border Control](https://vimeo.com/243958387)   [In Search of the World's Rarest Turtle](https://vimeo.com/285102429) From everyone at Biomeme to our long time collaborators, thank you for giving our platform a chance and congratulations on a strong publication! For those interested in conducting a study using our portable, field-ready platform, shoot us a message and let us know what you’re working on. [Let’s Talk!](https://biomeme.com/contact-us)   Citation: Tomaszewicz Brown A, McAloose D, Calle PP, Auer A, Posautz A, Slavinski S, et al. (2020) Development and validation of a portable, point-of-care canine distemper virus qPCR test. PLoS ONE 15(4): e0232044. [https://doi.org/10.1371/journal.pone.0232044](https://doi.org/10.1371/journal.pone.0232044) #### Subscribe Now Get the latest tips from Biomeme shipped right to your inbox ## Related Blog Posts [![](https://blog.biomeme.com/hubfs/DoD%20Partnership.png)](https://blog.biomeme.com/biomeme-on-the-front-lines) [Explained](https://blog.biomeme.com/tag/explained) ### Biomeme on the Front Lines The landscape of modern warfare is undergoing rapid transformation. This evolution demands preparedness not only against conventional threats but also against emerging chemical and biological... [Read More](https://blog.biomeme.com/biomeme-on-the-front-lines) [![](https://blog.biomeme.com/hubfs/Why%20Franklin_Blog.png)](https://blog.biomeme.com/why-we-call-it-the-franklin-biomeme) [Product Updates](https://blog.biomeme.com/tag/product-updates) ### Why Do We Call It Franklin? We have a very clear goal as a company: to improve global health for humans, animals, and the environment. 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[Read More](https://blog.biomeme.com/wcs-validates-canine-distemper-virus-qpcr-test-on-biomeme-platform) ![logo](https://blog.biomeme.com/hubfs/logo.svg) - [About](https://biomeme.com/about) - [Careers](https://biomeme.com/careers) - [Privacy Policy & Terms of Use](https://biomeme.com/privacy-policy-and-terms-of-use) - [Patents](https://biomeme.com/patents) 401 North Broad St Suite 222 Philadelphia, PA 19108 [Contact Us](https://biomeme.com/about/contact)